TB-500 (Thymosin Beta 4) 10 mg

TB-500 for research: identity and specifications

TB-500 (thymosin beta-4, also catalogued as timbetasin) is a synthetic 43-amino-acid, N-acetylated peptide corresponding to thymosin beta-4, an actin-regulating protein. Supplied as a 10 mg vial of lyophilized powder, with no fillers, carriers or excipients, at ≥98% purity by HPLC, with the lot-specific value reported on the certificate of analysis.

Scientific background

Thymosin beta-4 is an abundant intracellular protein that binds monomeric actin (G-actin) and takes part in regulating cytoskeletal polymerization. In experimental models, the peptide is investigated for cell migration, actin cytoskeleton dynamics and signaling related to angiogenesis in endothelial and other cell cultures.

Preclinical literature describes its use in studies of tissue repair and remodeling in rodent models, including skin and cardiac tissue, always under controlled bench conditions and without extrapolating results to human or veterinary use.

• G-actin binding and cytoskeletal dynamics in in vitro assays
• Endothelial and other cell migration in culture
• Angiogenic signaling in experimental models
• Tissue repair and remodeling in rodent models

Technical specifications

Analytical quality and chain of custody

Every lot of TB-500 is synthesized in registered facilities, with independent analytical verification by third-party laboratories and full chain-of-custody documentation. The certificate of analysis (COA) for each lot can be reviewed before purchase, recording HPLC purity and the other quality-control parameters used to release the material.

Reconstitution and laboratory handling

As a bench procedure, the lyophilized vial is reconstituted with a known volume of a suitable solvent, added along the inner wall of the vial, followed by gentle swirling until fully dissolved, without vigorous shaking. The reconstituted solution should be kept refrigerated and protected from light, and used in controlled assays within the stability period validated by the laboratory. Handling must be performed only by qualified personnel, in a bench environment suitable for in vitro experimentation, with lot number and storage conditions documented according to good laboratory practice.

Frequently asked questions

What is the purity of the TB-500 supplied?

≥98% by HPLC, with the lot-specific value reported on the certificate of analysis (COA).

Are TB-500 and thymosin beta-4 the same compound?

In this catalog, both names refer to the same material, identified by CAS number 77591-33-4 and PubChem CID 16132341 (timbetasin). In the literature, the term TB-500 is also used for a shorter synthetic fragment of thymosin beta-4; the specifications of the material supplied are those recorded on the lot COA.

How should the compound be stored?

At -20 °C, protected from light and moisture, as a lyophilized powder until reconstitution.

Does the certificate of analysis come with the lot?

Yes, every lot has a matching COA that can be verified before purchase using the button on this page.

Who can purchase TB-500?

Sales are restricted to qualified researchers and institutions, for laboratory use only, as detailed in the research-use-only notice on this page.

See the peptide reference guide, the page on how every lot is tested by HPLC and COA and the molecular structure in the PubChem database.

Research use only (RUO). This material is supplied for in vitro laboratory research only. It is not a drug, dietary supplement or cosmetic, and it is not intended for human or veterinary use, or for the diagnosis or treatment of any condition. Sales are restricted to researchers and institutions.