L-Glutathione 600–1500 mg

L-Glutathione for research: identity and specifications

L-Glutathione (GSH, reduced glutathione) is the tripeptide gamma-L-glutamyl-L-cysteinyl-glycine (CAS 70-18-8), notable for the gamma peptide bond between glutamate and cysteine. Supplied as a lyophilized powder in 600 mg and 1500 mg vials, with no fillers, carriers or excipients, at ≥98% purity by HPLC and the lot-specific value reported on the certificate of analysis.

Scientific background

The free thiol of the cysteine residue makes glutathione a central molecule in redox biology. In cell culture, it is used to study thiol-disulfide exchange, the balance between reduced (GSH) and oxidized (GSSG) forms, and conjugation reactions catalyzed by glutathione S-transferases.

Research applications include redox buffering in in vitro systems and the response of cultured cells to reactive oxygen species, always under controlled bench conditions and without extrapolating results to human or veterinary use.

• Redox state and thiol-disulfide exchange in cell culture
• Reduced versus oxidized glutathione (GSH/GSSG) analysis
• Glutathione S-transferase conjugation reactions
• Cellular response to reactive oxygen species in in vitro models

Technical specifications

Analytical quality and chain of custody

Every lot of L-Glutathione is synthesized in registered facilities, with independent analytical verification by third-party laboratories and full chain-of-custody documentation. The certificate of analysis (COA) for each lot can be reviewed before purchase, recording HPLC purity and the other quality-control parameters used to release the material.

Reconstitution and laboratory handling

As a bench procedure, the lyophilized vial is reconstituted with a known volume of a suitable solvent, added along the inner wall of the vial, followed by gentle swirling until fully dissolved, without vigorous shaking. Because the thiol group is susceptible to oxidation, exposure to air and light should be minimized and the solution kept refrigerated and used in controlled assays within the stability period validated by the laboratory. Handling must be performed only by qualified personnel, in a bench environment suitable for in vitro experimentation, with lot number and storage conditions documented according to good laboratory practice.

Frequently asked questions

What is the purity of the L-Glutathione supplied?

≥98% by HPLC, with the lot-specific value reported on the certificate of analysis (COA).

What is the difference between glutathione, GSH and GSSG?

GSH is the reduced, thiol-containing form of glutathione and is the compound supplied here (PubChem CID 124886). GSSG is the disulfide-linked oxidized dimer, which forms when two GSH molecules are oxidized.

How should the compound be stored?

At -20 °C, protected from light and moisture, as a lyophilized powder until reconstitution.

Does the certificate of analysis come with the lot?

Yes, every lot has a matching COA that can be verified before purchase using the button on this page.

Who can purchase L-Glutathione?

Sales are restricted to qualified researchers and institutions, for laboratory use only, as detailed in the research-use-only notice on this page.

See the peptide reference guide, the page on how every lot is tested by HPLC and COA, and the molecular structure in the PubChem database.

Research use only (RUO). This material is supplied for in vitro laboratory research only. It is not a drug, dietary supplement or cosmetic, and it is not intended for human or veterinary use, or for the diagnosis or treatment of any condition. Sales are restricted to researchers and institutions.