NAD+ 1000 mg

NAD+ for research: identity and specifications

NAD+ (nicotinamide adenine dinucleotide, CAS 53-84-9) is a coenzyme essential to cellular metabolism, present endogenously in living cells and involved in redox reactions. Supplied as a 1000 mg vial, lyophilized powder, with no fillers, carriers or excipients, at ≥99% purity by HPLC, with the lot-specific value reported on the certificate of analysis.

Scientific background

In in vitro assays and rodent models, NAD+ is investigated for its role as an electron carrier in the mitochondrial respiratory chain and as a substrate consumed by enzymes such as the sirtuins, linked to the regulation of gene expression, and the PARPs, involved in DNA repair pathways. The decline of intracellular NAD+ levels is studied in models of cellular senescence and aging.

The compound is used as a reference standard in NAD+-dependent enzyme assays and in comparative studies with precursors such as nicotinamide riboside and NMN, always under controlled bench conditions and without extrapolating results to human or veterinary use.

• Electron transfer and mitochondrial bioenergetics in in vitro assays
• NAD+-dependent sirtuin and PARP activity
• NAD+ levels in models of cellular senescence and aging
• Comparative studies with NAD+ precursors such as NMN

Technical specifications

Analytical quality and chain of custody

Every lot of NAD+ is synthesized in registered facilities, with independent analytical verification by third-party laboratories and full chain-of-custody documentation. The certificate of analysis (COA) for each lot can be reviewed before purchase, recording HPLC purity and the other quality-control parameters used to release the material.

Reconstitution and laboratory handling

As a bench procedure, the lyophilized vial is reconstituted with a known volume of sterile water or another suitable solvent, added slowly along the inner wall of the vial, followed by gentle inversion until fully dissolved, without vigorous shaking. Because the molecule is sensitive to light, moisture and pH, the reconstituted solution should be kept refrigerated and protected from light, and used in controlled assays within the stability period validated by the laboratory. Handling must be performed only by qualified personnel, in a bench environment suitable for in vitro experimentation, with lot number and storage conditions documented according to good laboratory practice.

Frequently asked questions

What is the purity of the NAD+ supplied?

≥99% by HPLC, with the lot-specific value reported on the certificate of analysis (COA). The current lot, NAD10001225-J, shows 99.936%.

Are NAD+, nadide and coenzyme I the same compound?

Yes, they are synonyms for the same molecule, identified by PubChem CID 5892, CAS 53-84-9 and molecular formula C21H27N7O14P2.

How should the compound be stored?

At -20 °C, protected from light and moisture, in the original sealed container, as a lyophilized powder until reconstitution.

Does the certificate of analysis come with the lot?

Yes, every lot has a matching COA that can be verified before purchase using the button on this page.

Who can purchase NAD+?

Sales are restricted to qualified researchers and institutions, for laboratory use only, as detailed in the research-use-only notice on this page.

See the peptide reference guide, the page on how every lot is tested by HPLC and COA and the molecular structure in the PubChem database.

Research use only (RUO). This material is supplied for in vitro laboratory research only. It is not a drug, dietary supplement or cosmetic, and it is not intended for human or veterinary use, or for the diagnosis or treatment of any condition. Sales are restricted to researchers and institutions.