PE-22-28 10 mg

PE-22-28 for research: identity and specifications

PE-22-28 is a synthetic heptapeptide of sequence Gly-Val-Ser-Trp-Gly-Leu-Arg (GVSWGLR), corresponding to residues 22-28 of spadin, the propeptide released during maturation of sortilin (CAS 1234388-01-0). Made by solid-phase peptide synthesis and purified by HPLC, it is supplied as a 10 mg vial, lyophilized powder, with no fillers, carriers or excipients, at ≥98% purity by HPLC and the lot-specific value reported on the certificate of analysis.

Scientific background

In cortical and hippocampal preparations, PE-22-28 is investigated for selective inhibition of TREK-1 (KCNK2), a two-pore-domain potassium channel that contributes to resting membrane potential. The peptide is used to study neuronal excitability and synaptic plasticity in experimental models.

Because it is a short fragment of a longer propeptide, PE-22-28 is also used in structure-activity work comparing the activity of spadin and its fragments, always under controlled bench conditions and without extrapolating results to human or veterinary use.

• TREK-1 (KCNK2) potassium channel inhibition in cortical and hippocampal preparations
• Neuronal excitability in electrophysiology models
• Synaptic plasticity in experimental systems
• Structure-activity relationships of spadin fragments

Technical specifications

Analytical quality and chain of custody

Every lot of PE-22-28 is synthesized in registered facilities, with independent analytical verification by third-party laboratories and full chain-of-custody documentation. The certificate of analysis (COA) for each lot can be reviewed before purchase, recording HPLC purity and the other quality-control parameters used to release the material.

Reconstitution and laboratory handling

As a bench procedure, the lyophilized vial is reconstituted with a known volume of a suitable solvent, added along the inner wall of the vial, followed by gentle swirling until fully dissolved, without vigorous shaking. The reconstituted solution should be kept refrigerated and protected from light, and used in controlled assays within the stability period validated by the laboratory. Handling must be performed only by qualified personnel, in a bench environment suitable for in vitro experimentation, with lot number and storage conditions documented according to good laboratory practice.

Frequently asked questions

What is the purity of the PE-22-28 supplied?

≥98% by HPLC, with the lot-specific value reported on the certificate of analysis (COA).

Is PE-22-28 the same as spadin?

No. Spadin is the longer propeptide released during sortilin maturation; PE-22-28 is the heptapeptide fragment (GVSWGLR) corresponding to residues 22-28 of it.

How should the compound be stored?

At -20 °C, protected from light and moisture, as a lyophilized powder until reconstitution.

Does the certificate of analysis come with the lot?

Yes, every lot has a matching COA that can be verified before purchase using the button on this page.

Who can purchase PE-22-28?

Sales are restricted to qualified researchers and institutions, for laboratory use only, as detailed in the research-use-only notice on this page.

See the peptide reference guide, the page on how every lot is tested by HPLC and COA.

Research use only (RUO). This material is supplied for in vitro laboratory research only. It is not a drug, dietary supplement or cosmetic, and it is not intended for human or veterinary use, or for the diagnosis or treatment of any condition. Sales are restricted to researchers and institutions.